Genetic Tools, LLC, has developed a brand new, revolutionary Thermophilic Reverse Transcriptase:
1) A type A DNA polymerase from bacterium, which works best at 60-62oC, and easily overcome high GC RNA template
2) Highly efficient: as little as one unit per RT reaction,
3) Heat inactivated: ≥90oC for 2 min
4) Detection limit: as low as single digit copies of target RNA template
5) Mg++ reaction buffer compatible with Taq DNA polymerase
6) Particularly suitable to one-step realtime-fluorescence RT-PCR