Thermophilic Reverse Transcriptase:
1) A type A DNA polymerase from bacterium, which works best at 60-62°C, and easily overcome high GC RNA template
2) Highly efficient: as little as one unit per RT reaction,
3) Heat inactivated: ≥90°C for 2 min
4) Detection limit: as low as single digit copies of target RNA template
5) Mg++ reaction buffer compatible with Taq DNA polymerase
6) Particularly suitable to one-step realtime-fluorescence RT-PCR
Pyrophosphorolysis Activated Polymerization (PAP)
PAP is a next generation of nucleic acid amplification technology. Unlike traditional PCR, this technology introduces a brand new principle in which two distinct steps (pyrophosphorolysis and polymerization) are serially coupled by polymerase using a blocked primer.
Double Layer Column Technology
Double layer column is based on next generation of nucleic acid extraction technology. Unlike the conventional silica technology, it introduces a new principle in which two distinct membranes are serially coupled within a column: the first DEAE anion exchange membrane and the second silica membrane.