Intended use

• The 1-Step 2X Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four templates.

• The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.

Kit Characteristics

• The kit is designed for multiplex RT-PCR with TaqMan probes.

• For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.

• The RTase is easily heat-inactivated at ≥90°C for 1min.

• The RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers, ≥1 unit per 20μL of reaction.

• The RTase reversely-transcribes single digit copies of target RNA molecules consistently.

• The kit also contains Taq-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.

• Up to four pairs of gene-specific primers can be applied in one reaction.

• The concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1).

• The preferable PCR product size is ≤150bp.

• The kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.

User Manual

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