Intended use

• The 1-Step 2X RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with TaqMan probe.

• The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and RNA template.

Kit Characteristics

• The kit is designed for singleplex RT-PCR with TaqMan probe.

• For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.

• The RTase is easily heat-inactivated at ≥90°C for 1min.

• The RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction.

• The RTase reversely-transcribes single digit copies of target RNA molecules consistently.

• The kit also contains Taq-Probe DNA polymerase specially engineered for TaqMan probe, generating S-shaped curve.

• The concentrations of the primers and probe are variable depending on assay designs and thermocycling protocols (User Manual Table 1).

• The preferable PCR product size is ≤150bp.

• The kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.

User Manual

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