Intended Use

• The 2X qPCR Master Mix is used for real-time qualitative and quantitative qPCR with TaqMan probe.

• The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and DNA template.

Kit Characterizations

• The kit is designed for singleplex qPCR with TaqMan probe.

• This kit uses Taq-Probe DNA polymerase specially engineered for TaqMan probe, which increased 5’ to 3’ exonuclease efficiency produces S-shaped curve.

• The concentrations of the primers and probe are variable depending on specific assays and thermocycling protocols (User Manual Table 1).

• The preferable PCR product size is ≤150bp.

• The kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).

User Manual

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