Intended Use

• The 2X Multiplex qPCR Master Mix is used for real-time qualitative and quantitative multiplex qPCR with TaqMan probes for up to 4 targets.

• The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and  templates.

Kit Characterizations

• The kit is designed for multiplex qPCR with TaqMan probes.

• The kit uses Taq-Probe DNA polymerase specially engineered for TaqMan probes, which increases 5’ to 3’ exonuclease efficiency and produces S-shaped curve.

• Two to four pairs of gene-specific primers can be applied in one reaction.

• The concentrations of the primers and probes are variable depending on specific assays and thermocycling protocols (User Manual Table 1).

• The preferable PCR product size is ≤150bp.

• The kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).

User Manual

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