Cat. PAP 121, PAP Polymerase HY (High Yield)
Cat. PAP 121, PAP Polymerase HY (High Yield)
Intended use
This thermostable DNA-dependent DNA polymerase is used for real-time fluorescent pyrophosphorolysis activated polymerization (PAP) amplification with SYBR Green dye.
Characteristics
The PAP polymerase has 5’-3’ DNA polymerase and pyrophosphorolysis activities, but lacks 3’-5’ exonuclease and 5’-3’ exonuclease activities.
Particularly, the PAP polymerase can efficiently catalyze pyrophosphorolysis to remove dideoxynucleotides or acyclonucleotides from the 3’ ends of annealed primers.
In addition, the PAP polymerase exhibits minimal terminal transferase activity, which adds extra adenines to the 3'-end of a DNA molecule.
The PAP polymerase can detect a single copy of target DNA template.
It is particularly suitable to real-time qualitative and quantitative PAP with SYBR Green dye.