Intended use

This thermostable DNA-dependent DNA polymerase is used for real-time fluorescence pyrophosphorolysis activated polymerization (PAP) amplification with SYBR Green dye.

Characteristics

• The PAP polymerase has 5’-3’ DNA poly­merase and pyrophosphorolysis activities, but lacks 3’-5’ exonuclease and 5’-3’ exonuclease activities.

• Particularly, the PAP polymerase can efficiently catalyze pyrophosphorolysis to remove dideoxynucleotides or acyclonucleotides from the 3’ ends of annealed primers.

• In addition, the PAP polymerase exhibits minimal terminal transferase activity, which adds extra adenines to the 3'-end of a DNA molecule.

• The PAP polymerase can detect a single copy of target DNA template.

• It is particularly suitable to real-time qualitative and quantitative PAP with SYBR Green dye.

User Manual