Cat. PAP 124, PAP Taq-Probe Polymerase

Cat. PAP 124, PAP Taq-Probe Polymerase

$1,800.00

Intended use

This thermostable DNA-dependent DNA polymerase is used for real-time-fluorescent pyrophosphorolysis activated polymerization (PAP) for sinleplex or multiplex TaqMan probes.

Characteristics

  • The PAP polymerase is specially engineered for TaqMan probes, generating S-Shaped curves.

  • The PAP polymerase has 5’-3’ DNA poly­merase, pyrophosphorolysis and 5’-3’ exonuclease activities, but lacks 3’-5’ exonuclease activity.

  • Particularly, the PAP polymerase can efficiently catalyze pyrophosphorolysis to remove dideoxynucleotides or acyclonucleotides from the 3’ ends of annealed primers.

  • The PAP polymerase can detect a single copy of target DNA template.

  • It is particularly suitable to real-time qualitative and quantitative PAP and RT-PAP with sinleplex or multiplex TaqMan probes.

User Manual

Quantity:
Add To Cart